FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12901850
Sequences flagged as poor quality0
Sequence length50-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC95800.045.027687120
GAGCACA152200.029.840078120
AGAGCAC182200.026.414951120
AAGAGCA289350.017.485046120
GAAGAGC333700.015.43822120
GGAAGAG407150.014.242372120
TCGGAAG430250.013.542154118-119
ATCGGAA518200.012.795639120
GATCGGA515200.012.595042120
CGGAAGA498200.012.035282120
AGATCGG605000.011.285778120
TAGATCG132600.010.944465118-119
CTAGATC38700.010.111368118-119
TTAGATC56700.09.0207405116-117
AAGATCG282700.08.043549120
GAGATCG268750.07.268719120
TCTAGAT22901.03682396E-106.996567120
CCTAGAT16800.06.602529118-119
TAAGATC80650.06.4947386120
CAGATCG265050.06.347197118-119
Produced by FastQC (version 0.11.5)