FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12171423
Sequences flagged as poor quality0
Sequence length50-126
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC156550.062.21183120
GAGCACA241850.042.105278120
AGAGCAC321050.032.13103120
AAGAGCA464850.022.47644120
GAAGAGC510950.019.845713118-119
GGAAGAG610350.017.606808120
CACGTCT47350.016.370977120
CGGAAGA730450.014.902384120
TCGGAAG721050.014.834894118-119
CACACGT45750.013.902371120
ATCGGAA884600.013.129363120
GATCGGA916900.013.078722120
AGATCGG1045150.011.451731118-119
TAGATCG196800.011.143244120
CCTAGAT18250.010.89106118-119
CTAGATC61150.010.726239120
ACACGTC57100.010.094664120
GTCTAGA6505.9980484E-69.173559120
GCTAGAT24350.09.115063116-117
AAGATCG437650.08.780309120
Produced by FastQC (version 0.11.5)