FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13845368
Sequences flagged as poor quality0
Sequence length51-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1566650.013.376389120
GCGTCGG120550.010.451156120
TCATTAA510750.09.256144120
CGTATCA655750.09.020921120
GTATCAT644250.08.883952120
ATCATTA572800.08.756197120
CCGTATC749350.08.558649120
GGTCGCC1025900.08.421117120
TATCATT670000.08.255976120
CATTAAA513550.08.247726120
TGGTCGC1076550.08.169811120
GTGGTCG1158800.08.102483120
GCCGTAT822250.07.9676642120
CGCCGTA896800.07.940857120
GTCGCCG1046650.07.795603120
CGGTGGT1364450.07.765069120
GGTGGTC1243800.07.7272363120
TCGGTGG1436850.07.5408196120
CTCGGTG1555150.07.4725623120
TCGCCGT1000500.07.4296174120
Produced by FastQC (version 0.11.5)