FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15902918
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC238200.0109.12386120
GAGCACA394950.065.393555120
AGAGCAC538650.048.189438118-119
AAGAGCA796650.032.40876120
GAAGAGC907350.028.545942116-117
GGAAGAG1095500.023.331305118-119
CGGAAGA1274200.020.417608114-115
TCGGAAG1317550.019.334387114-115
ATCGGAA1538700.017.04752112-113
ACACGTC71950.016.526262120
GATCGGA1578100.016.214663112-113
ACGTCTG81000.015.543328120
CACACGT62600.015.502979120
AGATCGG1723900.015.249059110-111
CACGTCT68300.015.104614118-119
GGTCCTG37150.014.5440921
GGTCCCC13050.014.4710561
GGTCTAC36400.014.2673091
TAGATCG382100.013.970197110-111
GGTCCCT13300.013.80462551
Produced by FastQC (version 0.11.5)