FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15264619
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC126200.055.860195120
GAGCACA197000.041.49312120
AGAGCAC260050.029.370262118-119
AAGAGCA417200.020.609478120
GAAGAGC478300.017.063124120
GGAAGAG597000.014.434762120
TCGGAAG616900.014.073295120
CGGAAGA679350.012.770132120
GATCGGA773000.011.896393120
ATCGGAA737050.011.844807118-119
CTAGATC50300.010.987165120
AGATCGG860150.010.534164120
TAGATCG176600.09.778408116-117
AAGATCG442650.08.042749120
TTAGATC86450.07.805118120
GAGATCG333200.06.9997873118-119
CACACGT48800.06.76201118-119
CAAGATC240850.06.723711120
ACACGTC57450.06.5995936120
TCTAGAT30200.06.596444120
Produced by FastQC (version 0.11.5)