FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15630677
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC184450.078.87881120
GAGCACA296400.049.390224120
AGAGCAC403800.037.420452120
AAGAGCA614000.024.993382120
GAAGAGC706000.021.676445118-119
GGAAGAG871400.017.698126120
CGGAAGA1026050.015.651811120
TCGGAAG1041250.015.429982120
ATCGGAA1205000.013.409126118-119
GATCGGA1226200.013.204352120
AGATCGG1345500.011.949692116-117
TAGATCG365350.011.257718118-119
CTAGATC83000.010.294212116-117
CGATCTA24500.09.7789595
GAGATCG499000.09.441713116-117
TTAGATC127200.09.313838120
AAGATCG408250.08.926459120
CAGATCG477400.08.897921114-115
CCTAGAT29600.08.660236120
ATAGATC183800.08.017489116-117
Produced by FastQC (version 0.11.5)