FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12365787
Sequences flagged as poor quality0
Sequence length51-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT81700.030.1101324
TCCGATC86300.028.9223353
CGATCTA22000.028.0908835
CTTCCGA124400.021.414771
TTCCGAT176750.015.2079072
AAAAAAA1227150.013.313764120
GATCTAG12050.011.4522726
CGATCTC79000.011.0126485
GATCTAA20600.010.4854276
CGTATCA509200.09.556158120
CGCCGTA619500.09.065367120
GTATCAT509600.08.759804120
TGGTCGC796550.08.632217120
GGTCGCC747950.08.559388120
GCCGTAT598450.08.501955120
CCGTATC568450.08.433451120
GTCGCCG726100.08.362476120
GTGGTCG859850.08.35959120
TCATTAA389450.08.242386120
TCGCCGT691400.08.192066120
Produced by FastQC (version 0.11.5)