FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13695051
Sequences flagged as poor quality0
Sequence length51-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG65050.010.882543120
TGGTCGC562000.010.18375120
CGTATCA336250.010.098336120
GTGGTCG612700.010.006881120
GGTGGTC674200.09.903806120
GGTCGCC523600.09.761929120
GCCGTAT416250.09.52671120
GTCGCCG508850.09.443619120
AAAAAAA841950.09.398405120
CGCCGTA435250.09.386507120
TCGGTGG813700.09.179113120
CGGTGGT761600.09.113855120
TCGCCGT495200.09.086075120
CTCGGTG875950.08.84184120
TCTCGGT939550.08.824373120
ATCTCGG1015400.08.720571120
CCGTATC407700.08.358018120
GTATCAT369700.08.2272415120
TCATTAA278750.08.178327120
GATCTCG1107050.08.063644120
Produced by FastQC (version 0.11.5)