FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13695051
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC184850.089.74586120
GAGCACA297100.054.505165120
AGAGCAC406900.040.662365120
AAGAGCA624550.026.80896120
GAAGAGC692050.024.056345118-119
GGAAGAG871000.019.143354118-119
CGGAAGA1033050.016.236107116-117
TCGGAAG1032100.016.07356120
ATCGGAA1230550.013.928384120
GATCGGA1268100.013.648967120
CACACGT39150.012.925835120
AGATCGG1357150.012.6263895118-119
TAGATCG313500.011.46293114-115
AAGATCG499150.010.696498120
CTAGATC72050.010.637006116-117
ACACGTC40900.010.400302120
CACGTCT41550.010.237601120
CCGATCT92000.09.6038414
CGATCTA21100.09.5410415
GAGATCG470300.09.395889110-111
Produced by FastQC (version 0.11.5)