FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13761005
Sequences flagged as poor quality0
Sequence length78-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA353100.09.991419120
TAGATCT210850.09.731955120
AGTGTAG315750.09.330087120
GATCTCG202650.09.267132120
AAGAGTG431650.09.201821120
GTGTAGA322950.09.08492120
GTAGATC321000.09.065344120
AGAGTGT383000.09.054753120
AGATCTC204400.08.864896120
GTAGGGA674700.08.430343120
TAGGGAA640700.08.409481120
GTGTAGG790250.08.2909775120
CTCGGTG177150.08.162505120
TCGTGTA902050.08.061569120
TGTAGGG724600.08.05679120
ATCTCGG202950.08.041322120
TGTAGAT359350.08.031099120
GGGAAAG643100.07.6783676120
CGTGTAG843650.07.659509120
AGGGAAA705350.07.5536327120
Produced by FastQC (version 0.11.5)