FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13761005
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC140450.059.252556120
GAGCACA203100.041.163097120
AGAGCAC269250.032.587177120
AAGAGCA429450.020.712717120
GAAGAGC488850.018.469446120
GGAAGAG617850.015.540733120
TCGGAAG714200.014.585344120
CGGAAGA724350.013.466772120
ATCGGAA848700.012.799059120
GATCGGA838250.012.676904118-119
AGATCGG930000.011.494672118-119
TAGATCG224800.010.70653120
GAGATCG365300.09.708649120
AAGATCG307000.09.395352120
CTAGATC55300.09.326381120
CAGATCG294800.08.704239120
TTAGATC87200.08.616259120
CCTAGAT23150.08.526365120
ACACGTC64850.07.9529505120
CACACGT54250.07.7463555120
Produced by FastQC (version 0.11.5)