FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12185629
Sequences flagged as poor quality0
Sequence length50-126
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC190900.0179.851120
GAGCACA333650.0103.09158120
AGAGCAC457550.075.2442118-119
AAGAGCA663850.050.0973118-119
GAAGAGC722300.047.246044116-117
CACGTCT43950.039.246120
ACGTCTG49250.039.112797120
GGAAGAG850450.038.65376116-117
CGTCTGA47650.036.462788120
CCGATCT13350.035.8002364
ACACGTC47250.035.705627120
CGGAAGA985500.034.685413114-115
GCACACG44350.032.646595120
CACACGT45000.031.335684118-119
TCGGAAG1090600.030.182613114-115
GAGCACC8850.028.452501120
ATCGGAA1261650.027.377876112-113
TCCGATC17700.027.2700043
GAACTCC32600.026.648033120
GATCGGA1304250.025.2963112-113
Produced by FastQC (version 0.11.5)