FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12432022
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC113050.053.448967120
GAGCACA163350.038.289803120
AGAGCAC219400.031.04007120
AAGAGCA349600.019.479952120
GAAGAGC403550.018.468922120
GGAAGAG486300.015.27486120
TCGGAAG558600.014.404113120
CGGAAGA586100.013.866722120
GATCGGA676050.012.834251120
ATCGGAA666150.012.256633118-119
AGATCGG772750.011.575553120
TAGATCG168400.010.41597118-119
CTAGATC43150.09.981683118-119
TTAGATC64250.09.47255118-119
AGTGAGT23650.08.7926181
CCTAGAT18100.08.276926116-117
AAGATCG335000.08.170719118-119
GAGATCG332800.07.840207120
CAGATCG322450.07.72405120
GTGAGTA27400.07.57816842
Produced by FastQC (version 0.11.5)