FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14864105
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC207600.099.99458120
GAGCACA363450.058.186832120
AGAGCAC496850.044.59407120
AAGAGCA747600.029.180515118-119
GAAGAGC861900.025.412123118-119
GGAAGAG1030850.021.1475116-117
CGGAAGA1188400.018.577799116-117
TCGGAAG1221400.017.842314114-115
ATCGGAA1400750.015.838462114-115
CACACGT44400.015.739753120
GATCGGA1448300.015.123643112-113
AGATCGG1597950.013.846456112-113
TAGATCG422250.012.883667110-111
CTAGATC102250.012.737937110-111
CACGTCT55600.012.426325120
GAGATCG585200.011.603326112-113
CAGATCG532900.011.363515110-111
ACACGTC55450.010.59824118-119
AAGATCG422000.010.341269110-111
TTAGATC128900.010.320018110-111
Produced by FastQC (version 0.11.5)