FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12344594
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC147500.071.70137120
GAGCACA229950.044.04332120
AGAGCAC311550.034.3542120
AAGAGCA468900.022.488117118-119
GAAGAGC528500.019.85723118-119
GGAAGAG662650.016.061037120
TCGGAAG765200.014.179584120
CGGAAGA790100.013.935911120
CACACGT44750.013.304071120
ATCGGAA910700.012.252037120
GATCGGA925200.011.937105120
AGATCGG1002000.011.077332118-119
TAGATCG219300.010.470106116-117
CTAGATC55600.010.158857116-117
ACACGTC47950.09.905066120
CACGTCT47150.09.08120
AAGATCG423400.08.578999120
GAGATCG367050.08.149496116-117
TTAGATC92550.08.016956112-113
CAGATCG354200.07.4355073112-113
Produced by FastQC (version 0.11.5)