FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15807708
Sequences flagged as poor quality0
Sequence length51-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT86850.014.7827574
TCCGATC95950.014.5068693
GCGTCGG85350.014.058377120
AAAAAAA1684850.012.971991120
CGATCTA22250.012.4033195
CTTCCGA134050.010.4288131
TCATTAA595650.09.981395120
CGTATCA771000.09.594391120
CGCCGTA981400.09.408086120
GCCGTAT926250.09.372361120
GGTCGCC1215600.09.347554120
ATCATTA674100.09.25588120
TCGCCGT1092000.09.119986120
GTATCAT758050.09.117239120
GTCGCCG1164550.09.072144120
CCGTATC866900.08.9136505120
TGGTCGC1295100.08.833973120
GTGGTCG1402650.08.828137120
GGTGGTC1514800.08.784458120
TCTCGGG36250.08.606053120
Produced by FastQC (version 0.11.5)