FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15807708
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC249350.0117.28785120
GAGCACA419400.068.05773120
AGAGCAC578650.050.106895118-119
AAGAGCA853300.033.872395118-119
GAAGAGC973250.029.67968116-117
GGAAGAG1181950.024.318022116-117
CGGAAGA1405050.020.697046114-115
TCGGAAG1446200.019.865326114-115
ATCGGAA1669550.017.416176112-113
GATCGGA1717900.016.751871112-113
CCGATCT88550.016.7428554
TCCGATC97600.016.1123853
AGATCGG1856300.015.615452110-111
CACGTCT55800.014.917057120
CACACGT53300.014.315336120
TAGATCG399750.014.022777112-113
GAGATCG625050.012.922139110-111
TTAGATC154200.012.848966110-111
CAGATCG603350.012.603658110-111
CTAGATC87950.012.569798112-113
Produced by FastQC (version 0.11.5)