FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13232673
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC140400.079.52099120
GAGCACA231900.047.703236120
AGAGCAC323750.035.940174120
AAGAGCA514400.022.87188120
GAAGAGC560750.021.01848118-119
GGAAGAG672250.017.663794120
CGGAAGA787950.015.083533118-119
TCGGAAG800700.014.736809118-119
ATCGGAA952600.012.8522120
GATCGGA947950.012.595249118-119
AGATCGG1025350.011.607872118-119
TAGATCG247000.011.493755120
CTAGATC52500.010.139658116-117
CAGATCG383000.09.354618120
GAGATCG383650.09.223624116-117
TTAGATC95600.08.99681112-113
AAGATCG307300.08.40774112-113
CACACGT35500.08.074194118-119
TGAGATC190700.08.000361110-111
ATAGATC144400.07.892505120
Produced by FastQC (version 0.11.5)