FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15474296
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC146850.062.598267120
GAGCACA222600.040.605633120
AGAGCAC288000.033.09747120
GAAGAGC507900.019.49915120
AAGAGCA498200.019.480196120
GGAAGAG630800.016.014906120
CGGAAGA727450.014.327446120
TCGGAAG731400.014.039548118-119
GATCGGA884150.012.280842120
ATCGGAA890800.012.016573120
AGATCGG963650.011.236611118-119
CACACGT39750.010.797472120
TAGATCG252000.010.621667118-119
CTAGATC62700.08.990824120
AAGATCG445350.08.86061120
ACACGTC45250.08.352526120
TTAGATC129650.08.115048118-119
GAGATCG293900.08.064668120
CAGATCG290900.07.6413507120
TGAGATC152600.07.010457120
Produced by FastQC (version 0.11.5)