FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19143797
Sequences flagged as poor quality0
Sequence length59-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG83500.018.896511120
CGTATCA625550.011.480068120
GCCGTAT765900.011.373835120
CCGTATC699650.011.156008120
CGCCGTA814950.011.108889120
TGGTCGC1142050.010.910979120
GTCGCCG992650.010.770204120
GGTCGCC1041950.010.727001120
GTATCAT633300.010.60066120
TCTCGGG31000.010.450677120
TCGCCGT932550.010.222664120
GTGGTCG1248750.010.13723120
GGTGGTC1364650.09.944516120
AAAAAAA1583000.09.554419120
CTCGGTG1759500.09.390463120
CGGTGGT1506600.09.226573120
TCGGTGG1629050.09.195948120
TCTCGGT1905400.08.85404120
TCATTAA563500.08.77295120
ATCTCGG2066600.08.64531120
Produced by FastQC (version 0.11.5)