FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12922833
Sequences flagged as poor quality0
Sequence length53-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1496400.014.6534195120
CGTATCA649100.09.418069120
TCATTAA497350.09.167497120
GTATCAT640850.08.884011120
CCGTATC744900.08.778651120
ATCATTA575350.08.727556120
CATTAAA487800.08.645952120
CGCCGTA845650.08.506048120
GCGTCGG93450.08.474107120
GGTCGCC1001500.08.296561120
GCCGTAT789550.08.236618120
TCGCCGT942250.08.175196120
TGGTCGC1051100.07.876519120
GTGGTCG1126700.07.7793126120
ATTAAAA570100.07.67142120
GTCGCCG987650.07.6445065118-119
TATCATT673950.07.5664215120
GGTGGTC1204850.07.4589577120
CTCGGTG1464000.06.941792120
CGGTGGT1311500.06.879848120
Produced by FastQC (version 0.11.5)