FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12384650
Sequences flagged as poor quality0
Sequence length63-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG91100.09.353307120
ATCTCGG411700.09.269824120
TAGATCT469000.09.058486120
AGATCTC451450.08.932126120
TCTCGGT369150.08.810319120
GTGTAGA629300.08.79161120
GTGGTCG258850.08.693173120
CTCGGTG360400.08.607974120
AGTGTAG652000.08.58676120
GATCTCG450050.08.546582120
GGTGGTC286750.08.516991120
GTAGATC619500.08.465748120
TCGGTGG333150.08.321419120
TGTAGAT637950.08.145663120
AGAGTGT750000.08.120822120
GAGTGTA694200.08.064775120
AAGAGTG824100.07.958578120
CGGTGGT331000.07.94038120
TGGTCGC234800.07.4879813120
GGTCGCC247000.07.3124814120
Produced by FastQC (version 0.11.5)