FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12384650
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC153750.076.69798120
GAGCACA239450.046.516228120
AGAGCAC321550.036.253986120
AAGAGCA489150.024.342096120
GAAGAGC556350.021.290754118-119
GGAAGAG667350.017.963352120
CGGAAGA789450.015.244841120
TCGGAAG805200.015.038753120
ATCGGAA937250.013.315585120
GATCGGA970200.012.953703120
AGATCGG1072750.011.728991118-119
TAGATCG237850.011.1659118-119
CTAGATC60100.010.360979114-115
TTAGATC95250.09.576027110-111
AAGATCG449700.09.4458275114-115
GAGATCG364050.08.996249116-117
TAAGATC165200.08.77481120
ACACGTC50400.08.695451120
CACACGT43850.08.456728120
CACGTCT47050.08.155772118-119
Produced by FastQC (version 0.11.5)