FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15356981
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC207150.084.7414120
GAGCACA334950.049.70922120
AGAGCAC459350.037.271717118-119
AAGAGCA694900.025.478285120
GAAGAGC757150.023.075998120
GGAAGAG922250.019.10092120
CGGAAGA1115800.015.763603118-119
TCGGAAG1147500.015.283449118-119
CACGTCT55950.015.179363120
CACACGT54050.013.938915120
ATCGGAA1374150.013.3328085120
GATCGGA1405100.012.758815116-117
ACGTCTG66550.012.452867120
AGATCGG1560250.012.36148120
ACACGTC60450.012.156659118-119
TAGATCG347350.010.578236116-117
CTAGATC85950.010.18051114-115
GAGATCG543950.010.072717118-119
AAGATCG577850.09.523198118-119
CAGATCG487500.08.97451114-115
Produced by FastQC (version 0.11.5)