FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15478267
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC165600.069.34096120
GAGCACA251800.045.655922120
AGAGCAC340750.035.864723120
AAGAGCA537950.022.668106120
GAAGAGC591050.020.980335120
GGAAGAG717500.017.174587118-119
CGGAAGA835100.014.992349120
TCGGAAG837550.014.9167385120
GATCGGA1003300.012.942831120
ATCGGAA984800.012.819968118-119
AGATCGG1114150.011.6670475120
TAGATCG271300.010.678693118-119
CTAGATC71350.09.966357112-113
AAGATCG399600.08.84642118-119
GAGATCG407650.08.824044120
TTAGATC113800.08.349783118-119
CAGATCG412500.08.134836118-119
CCTAGAT26800.07.4429364120
ATAGATC125400.07.1269903118-119
TGAGATC190000.07.068961120
Produced by FastQC (version 0.11.5)