FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14299220
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC130200.059.54974120
GAGCACA198450.042.341675120
AGAGCAC257700.031.783268120
AAGAGCA422800.019.952715118-119
GAAGAGC452950.018.072016118-119
GGAAGAG568500.014.918314116-117
TCGGAAG646200.014.107591120
CGGAAGA680350.013.0214815120
GATCGGA768600.011.82749118-119
ATCGGAA789550.011.562514120
TAGATCG223350.011.283495120
AGATCGG829550.010.981732120
CTAGATC57050.09.393208116-117
AAGATCG347350.08.106816120
TTAGATC98800.07.892157118-119
CAGATCG332500.07.7148023120
CCCTAGA7550.00778299067.6637106120
GAGATCG293850.07.548088120
ATAGATC129800.06.2792163118-119
GCTAGAT25400.05.812242108-109
Produced by FastQC (version 0.11.5)