FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15311214
Sequences flagged as poor quality0
Sequence length50-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC275000.0111.762535120
GAGCACA459550.066.49261120
AGAGCAC632550.048.439938118-119
AAGAGCA942050.032.58682118-119
GAAGAGC1025550.029.647583116-117
CACGTCT84000.024.053534120
GGAAGAG1266700.023.915733116-117
CACACGT83050.022.07801120
ACACGTC85000.021.990377120
CGGAAGA1482650.020.552006114-115
TCGGAAG1543200.019.541311114-115
GCACACG91050.017.850504118-119
ATCGGAA1824450.016.967243112-113
GATCGGA1874950.016.29472112-113
ACGTCTG105850.015.556548120
CGTCTGA125200.015.356131120
AGATCGG2029400.015.352223110-111
TAGATCG515550.014.246693110-111
CTAGATC119050.013.092671108-109
CAGATCG717550.012.804354110-111
Produced by FastQC (version 0.11.5)