FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12940897
Sequences flagged as poor quality0
Sequence length60-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT202000.010.662918120
AGTGTAG291050.09.977247120
GAGTGTA314200.09.853182120
GTGTAGA285300.09.757738120
AGAGTGT348550.09.346903120
AAGAGTG397650.09.052806120
TAGGGAA580150.08.997294120
GTAGATC301700.08.829592120
TCGGTGG156750.08.765181120
AGATCTC206100.08.558591120
GATCTCG216650.08.363368120
GTAGGGA606850.08.27546118-119
TGTAGGG656000.08.258799120
GTGGTCG109300.08.069194120
TCTCGGT170900.08.004344120
CGTGTAG758350.07.9590507120
TCGTGTA814450.07.889655120
TGGTCGC99450.07.8428073120
GTGTAGG720300.07.8214116120
AGGGAAA636800.07.8106008120
Produced by FastQC (version 0.11.5)