FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15247157
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC170100.076.61761120
GAGCACA262950.048.77943120
AGAGCAC364550.036.654568120
AAGAGCA562800.023.567423118-119
GAAGAGC627350.021.268278118-119
GGAAGAG766300.017.34453116-117
CGGAAGA918450.014.713446120
TCGGAAG921650.014.432138114-115
GATCGGA1063250.012.71614120
ATCGGAA1070200.012.557104114-115
AGATCGG1153850.011.600944112-113
TAGATCG298050.010.787538120
CTAGATC74400.010.006627114-115
GAGATCG392450.09.5201645114-115
TTAGATC115000.09.457269118-119
AAGATCG379850.09.197739110-111
CAGATCG396000.08.848628110-111
ATAGATC145350.07.57941110-111
TGAGATC158900.07.479762120
TAAGATC153600.07.306375110-111
Produced by FastQC (version 0.11.5)