FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12778272
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC126050.060.847866120
GAGCACA180750.039.54769120
AGAGCAC239950.031.104366118-119
AAGAGCA392800.020.804947120
GAAGAGC429650.019.844933120
GGAAGAG525500.015.979514118-119
TCGGAAG594350.014.107341120
CGGAAGA611900.013.654808118-119
GATCGGA718000.012.180125120
ATCGGAA714700.012.155271120
AGATCGG800950.011.441315120
TAGATCG207850.010.596258120
CTAGATC47300.09.393915118-119
ACACGTC39250.09.352154120
CACACGT33800.09.335892120
GAGATCG302450.08.793725120
TTAGATC96100.08.376509120
AAGATCG314650.08.165895118-119
CACGTCT37550.07.717542120
CAGATCG280550.07.5060925120
Produced by FastQC (version 0.11.5)