FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14172189
Sequences flagged as poor quality0
Sequence length64-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA759850.010.728668120
GCGTCGG153350.09.355952120
GTATCAT328800.08.416723120
ATCTCGG848050.08.140486120
CGTATCA311550.07.982224118-119
TCGGTGG704850.07.963222120
GTGGTCG565800.07.9043694120
TGGTCGC516000.07.864496120
AAAGAGG155150.07.815801120
AGATCTC947600.07.6590543120
GTGTAGA1209150.07.6456504120
CTCGGTG778250.07.644137120
TCTCGGT776650.07.590319120
GGTGGTC640700.07.5424953120
GCCGTAT413300.07.422144120
TAGATCT971450.07.3968635120
GATCTCG953950.07.3437715120
GAGTGTA1319250.07.3260975120
CGCCGTA478000.07.2840686120
TCATTAA255400.07.2159147120
Produced by FastQC (version 0.11.5)