FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18243419
Sequences flagged as poor quality0
Sequence length51-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT304200.08.838188120
AGATCTC321950.08.798285120
GTGGTCG172900.08.781526120
GAGTGTA457550.08.761578120
AGTGTAG422700.08.617889120
GTGTAGA423950.08.49339120
GTAGATC411650.08.455598120
AGAGTGT513000.08.387764120
ATCTCGG295800.08.155908120
AAGAGTG584100.08.11679120
TGGTCGC160400.08.081533120
TAGGGAA795500.07.883527120
GTAGGGA826750.07.7815304120
GGTGGTC207250.07.644583120
TGTAGGG887450.07.546833120
TCTCGGT275600.07.534272120
GATCTCG322200.07.450388120
GCGTCGG88750.07.4382157120
TGTAGAT486050.07.4329286120
GGTCGCC166100.07.370636120
Produced by FastQC (version 0.11.5)