FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13229860
Sequences flagged as poor quality0
Sequence length50-126
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC179800.0160.01463120
GAGCACA312050.093.6325120
AGAGCAC439250.066.259514118-119
AAGAGCA667000.043.213535118-119
GAAGAGC740950.039.187958116-117
GGAAGAG874850.032.947453116-117
CGGAAGA1032750.027.965824114-115
TCGGAAG1064950.026.953266114-115
ATCGGAA1228250.023.579899112-113
GATCGGA1259900.022.823643112-113
CACGTCT41300.021.665384120
AGATCGG1341800.021.494282110-111
TAGATCG350200.019.622581110-111
ACGTCTG56550.018.662813120
CCCCCCG1801.1460674E-418.6523231
CAGATCG390250.018.402502110-111
GCACACG46550.018.236181120
CACACGT39050.018.213463120
ACACGTC42950.017.895075120
GAGATCG441750.017.503658110-111
Produced by FastQC (version 0.11.5)