FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14526104
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC163600.067.935135120
GAGCACA244650.044.07403120
AGAGCAC325050.034.112263120
AAGAGCA512850.022.431822120
GAAGAGC562550.020.554005120
GGAAGAG707800.017.373697120
TCGGAAG840500.014.6499195118-119
CGGAAGA862100.014.233968120
CACACGT47850.013.718956120
ATCGGAA1009050.012.611933120
GATCGGA1026350.012.342354120
AGATCGG1115600.011.937547120
CACGTCT47050.011.67281118-119
ACACGTC48200.011.057284120
TAGATCG284500.010.920116120
ACGTCTG60850.09.826727120
CTAGATC71100.09.507041120
GAGATCG386600.09.296974118-119
AAGATCG395500.09.153566120
CAGATCG369900.08.662519120
Produced by FastQC (version 0.11.5)