FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14998376
Sequences flagged as poor quality0
Sequence length52-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1707100.012.936653120
GCGTCGG91950.012.070772120
GTATCAT696400.09.321451120
CGTATCA705600.09.089378120
GCCGTAT846800.08.941154120
CGCCGTA893400.08.736679120
GGTCGCC1085850.08.713184120
CCGTATC795800.08.586868120
TGGTCGC1148300.08.578925120
CATTAAA564450.08.375598120
TCGCCGT981350.08.296042120
GTGGTCG1237750.08.27885120
GTCGCCG1048750.08.111839120
TCTCGGG37150.08.0746975120
TCATTAA585600.08.052608120
ATCATTA656000.07.9200716120
TATCATT758850.07.795357120
GGTGGTC1325050.07.7560425120
CGGTGGT1443000.07.4006305120
CTCGGTG1617500.07.32551120
Produced by FastQC (version 0.11.5)