FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12661031
Sequences flagged as poor quality0
Sequence length67-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGG239600.010.916704120
TGTAGGG211900.010.418585120
CGTGTAG258150.010.387888120
GTAGGGA196600.010.313951120
TCGTGTA269850.010.182042120
GTCGTGT299600.010.112101120
AGAGTGT94550.09.390572120
GAGTGTA80550.09.384191120
TAGGGAA190850.09.367332120
AGTGTAG73500.09.304849120
AAGAGTG115300.08.689184120
CGTCGTG376200.08.659102120
AGAGCGT484550.08.493318120
TAGATCT54750.08.4372015120
AGCGTCG417850.08.413352120
GCGTCGT407850.08.16365120
GAGCGTC468550.07.963909120
AAGAGCG535250.07.8345194120
GTGTAGA88200.07.822059120
TCGGAAG701050.07.5904317120
Produced by FastQC (version 0.11.5)