FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12661031
Sequences flagged as poor quality0
Sequence length50-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC80450.046.183002120
GAGCACA125000.029.772346120
AGAGCAC155300.026.959023120
AAGAGCA249850.017.76144120
GAAGAGC310250.015.566276120
TCGGAAG404200.013.841061120
GATCGGA455050.012.724816120
GGAAGAG398850.012.52276120
ATCGGAA471450.011.821261118-119
AGATCGG518350.011.348011120
TAGATCG129850.011.195439118-119
CGGAAGA511650.011.077902120
CTAGATC36250.09.118119120
TTAGATC61650.08.886065118-119
AAGATCG227600.08.283196120
GAGATCG237400.08.0443945120
CAGATCG209200.06.0419602118-119
ATAGATC65900.06.0373654120
GCTAGAT20702.564775E-105.9139705120
CCTAGAT22900.05.880398120
Produced by FastQC (version 0.11.5)