FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14983814
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC145200.074.2569120
GAGCACA237800.047.196793120
AGAGCAC321050.036.250885120
AAGAGCA534900.022.188961120
GAAGAGC579500.020.844938120
GGAAGAG708650.017.743074120
CGGAAGA827850.015.031503118-119
TCGGAAG837400.014.676869120
GATCGGA1017550.013.424797120
ATCGGAA993600.012.594959120
TAGATCG322700.011.981022120
AGATCGG1119200.011.84652120
CTAGATC73850.09.817773118-119
CAGATCG384050.09.672664120
GAGATCG409150.09.06883118-119
AAGATCG365150.08.7869625118-119
TTAGATC137350.08.718372116-117
CACACGT39300.07.847052118-119
ATAGATC163800.07.510842114-115
TGAGATC173000.07.233729120
Produced by FastQC (version 0.11.5)