FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14270141
Sequences flagged as poor quality0
Sequence length53-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG96100.09.676587120
GTGGTCG313950.08.733124120
AGATCTC536950.08.603413120
CTCGGTG421300.08.444563120
TAGATCT552550.08.338799120
GGTGGTC341000.08.11074120
TCTCGGT448000.08.00824120
TCGGTGG411850.07.939101120
ATCTCGG501750.07.84387120
AGTGTAG741050.07.820662120
TGGTCGC286650.07.80676120
GATCTCG527200.07.772473120
GGTCGCC291450.07.760527120
GTGTAGA719800.07.6848073120
GAGTGTA790700.07.564798120
AGAGTGT852700.07.521342120
GTAGATC718950.07.3684464120
TGTAGAT740650.07.241665120
AAGAGTG928400.07.1148553120
CGGTGGT408950.06.8804455120
Produced by FastQC (version 0.11.5)