FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14270141
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC158450.074.3074120
GAGCACA257550.047.214294120
AGAGCAC341750.035.054504118-119
AAGAGCA514250.024.319183120
GAAGAGC604150.020.865639120
GGAAGAG749500.016.871817118-119
CGGAAGA892100.014.749919120
TCGGAAG896000.014.470298120
ATCGGAA1041500.013.094206120
GATCGGA1054050.012.606309118-119
AGATCGG1168750.011.679937120
TAGATCG266400.010.830136116-117
CTAGATC66150.010.262822120
TTAGATC102900.09.669518116-117
AAGATCG513700.09.160321114-115
GAGATCG392700.09.007854116-117
CAGATCG361350.08.841168120
TCTAGAT32850.08.5096245120
CCTAGAT21950.08.49024120
ACACGTC52800.08.445733120
Produced by FastQC (version 0.11.5)