FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13087804
Sequences flagged as poor quality0
Sequence length50-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC156700.069.03589120
GAGCACA240100.045.60976120
AGAGCAC322800.034.02772120
AAGAGCA506050.021.922499118-119
GAAGAGC544800.021.675156120
GGAAGAG663700.017.296253118-119
TCGGAAG781700.015.557146120
CGGAAGA774350.015.318417120
ATCGGAA944450.012.830566118-119
GATCGGA960400.012.333632118-119
AGATCGG1047400.011.610675120
TAGATCG228900.011.009048120
CTAGATC56950.09.632002116-117
ACACGTC45300.09.540507120
CACGTCT47800.09.458829120
GAGATCG418550.09.07079120
CACACGT43250.08.916578120
AAGATCG361850.08.590321116-117
ACGTCTG62000.08.472113120
TTAGATC98500.08.471569116-117
Produced by FastQC (version 0.11.5)