FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14727370
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC145650.069.05006120
GAGCACA221200.045.127987120
AGAGCAC283200.036.675114120
AAGAGCA457150.023.702005120
GAAGAGC523700.021.118698120
GGAAGAG620500.017.926306118-119
CGGAAGA751350.015.227915120
TCGGAAG760900.014.884098118-119
ATCGGAA915200.013.049448120
GATCGGA930500.012.9152975120
AGATCGG1029350.011.642105118-119
TAGATCG257800.010.826826120
CTAGATC69900.09.795161118-119
CACGTCT45950.09.282493120
TTAGATC108700.09.155823120
CACACGT46200.09.070292120
AAGATCG432450.08.772984120
GAGATCG405150.08.135486116-117
CCTAGAT22600.07.6154337120
TAAGATC169650.07.586646120
Produced by FastQC (version 0.11.5)