FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13288468
Sequences flagged as poor quality0
Sequence length50-126
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC173000.0172.66345120
GAGCACA311050.096.871124120
AGAGCAC442900.068.26055118-119
AAGAGCA663300.045.70426118-119
GAAGAGC754400.040.052185116-117
GGAAGAG889400.034.192837116-117
CGGAAGA1048350.028.975958114-115
TCGGAAG1090150.027.826836114-115
ATCGGAA1248100.024.354177112-113
GATCGGA1284000.023.673248112-113
AGATCGG1368250.021.93912110-111
TAGATCG328400.020.21441110-111
GAGATCG530250.018.762318110-111
CTAGATC73100.016.96145110-111
CAGATCG460000.016.751938112-113
AAGATCG366450.016.350866110-111
TCCGATC70550.016.333733
TTAGATC132900.016.070145108-109
CCGATCT69800.015.8952574
TGAGATC230150.015.595045108-109
Produced by FastQC (version 0.11.5)