FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10635964
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC121500.069.58066120
GAGCACA191300.044.300068120
AGAGCAC251000.034.227364120
AAGAGCA382100.023.613394120
GAAGAGC438750.019.963566118-119
GGAAGAG512450.017.072184120
CGGAAGA607300.014.4846116-117
TCGGAAG600900.014.439353116-117
ATCGGAA696850.012.6638365114-115
GATCGGA698950.012.546237120
AGATCGG777400.011.435663112-113
CTAGATC43100.010.969084110-111
TAGATCG164850.010.722114120
TTAGATC67700.09.4120245114-115
AAGATCG299550.09.252257110-111
GAGATCG297750.09.215211118-119
TAAGATC104700.08.540089110-111
CAGATCG230050.08.427793120
GCTAGAT17100.08.41352118-119
CACACGT45050.08.211994120
Produced by FastQC (version 0.11.5)