FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14086242
Sequences flagged as poor quality0
Sequence length54-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGA324050.010.94154120
GAGTGTA360600.010.597824120
TAGATCT217800.010.439612120
AGTGTAG328650.010.386797120
AGATCTC224550.010.099078120
AGAGTGT403900.010.055827120
GTAGATC346600.09.814259120
TGTAGAT365950.09.770741120
AAGAGTG452800.09.44683120
TAGGGAA711400.09.251151120
TCTCGGT198900.09.169418120
GGGAAAG690600.09.008556120
TGTAGGG825150.08.979188120
GTGTAGG899000.08.795464120
GTAGGGA752650.08.624565120
ATCTCGG219500.08.554858120
GATCTCG257150.08.282184120
AGGGAAA754050.08.250525120
GAAAGAG643150.08.236666120
CGTGTAG958350.08.019143120
Produced by FastQC (version 0.11.5)