FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14086242
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC168350.060.014004120
GAGCACA254300.042.230568120
AGAGCAC333050.033.055096120
AAGAGCA511200.022.264343120
GAAGAGC565900.019.669619118-119
GGAAGAG676100.017.299599120
CGGAAGA802850.015.16044120
TCGGAAG803650.014.929558120
GATCGGA991350.012.932144120
ATCGGAA968550.012.633101118-119
ACACGTC48600.012.026581120
AGATCGG1128850.011.374009120
CACACGT42550.011.170412120
CACGTCT41650.010.949149120
TTAGATC105700.010.69485120
TAGATCG252350.010.499236118-119
CTAGATC66200.09.896368114-115
AAGATCG424500.08.821204120
GAGATCG444800.08.606339120
CAGATCG419900.07.801199118-119
Produced by FastQC (version 0.11.5)