FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12419678
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC193600.096.89171120
GAGCACA322500.057.688717120
AGAGCAC430150.044.567223120
AAGAGCA623050.030.535816118-119
GAAGAGC721050.026.312716118-119
GGAAGAG879500.021.516495116-117
CGGAAGA1025950.018.488451116-117
TCGGAAG1051700.017.897072114-115
ATCGGAA1227100.015.628797114-115
GATCGGA1270700.014.956287112-113
CACGTCT64200.014.231392120
AGATCGG1381100.014.0036955112-113
TAGATCG328900.013.115266112-113
CTAGATC65150.012.534689112-113
CACACGT65100.011.861317118-119
GAGATCG543050.011.8143835110-111
TTAGATC88350.010.981929110-111
CGTCTGA93350.010.740174120
ACACGTC71900.010.57068120
CAGATCG525300.010.543556110-111
Produced by FastQC (version 0.11.5)