FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18167798
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT137000.036.8762634
TCCGATC148950.035.0053673
CGATCTA35650.031.472955
CTTCCGA189000.028.1705511
TTCCGAT267200.019.940312
CGATCTC92150.016.212855
TCTCGGG28750.015.045568120
AAAAAAA4419800.015.033719120
CGGTGGG23700.014.449089120
CGATCTT110450.012.6574325
CGATCTG107050.012.3308055
GATCTAG26600.011.7293396
GATCTAA36450.011.522656
TCATTAA1495300.09.630607120
GTATCAT1852650.09.575997120
CATTAAA1429300.09.516275120
GGCTAGC5050.0045016829.50451859
GCGTCGG95800.09.469464120
ATCATTA1651200.09.2743635120
CCGTATC2100600.09.169256120
Produced by FastQC (version 0.11.5)