FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13619889
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC126900.063.632282120
GAGCACA188950.040.87776120
AGAGCAC252500.032.62367120
AAGAGCA408350.021.064196120
GAAGAGC446150.020.153889120
GGAAGAG570300.015.743738120
TCGGAAG656550.014.586547120
CGGAAGA678300.013.764174120
ATCGGAA785650.012.11828118-119
GATCGGA789350.012.008054118-119
AGATCGG853450.011.102298116-117
TAGATCG192050.010.291456120
CTAGATC48400.09.315986116-117
TTAGATC86350.09.166148116-117
AAGATCG394950.08.435051116-117
GAGATCG303350.08.294392120
CCTAGAT19300.07.7479734120
GCTAGAT23850.07.2080326110-111
CAGATCG307100.06.9228587120
TAAGATC162550.06.719545120
Produced by FastQC (version 0.11.5)