FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15104372
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC153950.067.37666120
GAGCACA235300.047.48222120
AGAGCAC317850.034.131027118-119
AAGAGCA500650.022.791494120
GAAGAGC572700.020.097307120
GGAAGAG701350.016.486225120
TCGGAAG832150.014.387425120
CGGAAGA844450.013.825464118-119
GATCGGA987150.012.396227120
ATCGGAA984700.012.071243120
AGATCGG1076350.011.258362120
TAGATCG279250.010.724277118-119
CTAGATC63750.010.370174120
TTAGATC96200.09.414814120
GAGATCG418250.08.590661118-119
GCCTAGA10854.5619017E-68.530305120
GACCTAG4808.764408E-58.26367118-119
CCCTAGA8054.14002E-98.212064110-111
CAGATCG427800.07.9894342120
CCTAGAT23050.07.8869066112-113
Produced by FastQC (version 0.11.5)